Organic Light?Emitting Diode Based Fluorescence Sensing System for DNA Detection

Conventional fluorescence sensing equipment for disease detection is expensive and bulky, restricting access of patients to accurate diagnosis. Organic light-emitting diodes (OLEDs) have the potential enable compact compatible with point-of-care (POC) testing. However, limited brightness broad emission spectra OLEDs can be a challenge achieving good sensitivity. Here, co-host microcavity narrowed spectra, high conductivity, are developed sensing. The driven in pulsed mode higher stable light output. To sense presence single-stranded DNA (ssDNA), two complementary ssDNA labelled Cyanine dyes – Cy3 (ssDNA-Cy3) or Cy5 (ssDNA-Cy5) used form Förster resonance energy transfer (FRET) pair when hybridized. dye-labelled then excited by OLED at excitation wavelength, detected wavelength Cy5. As result, device shows very sensitivity that detect as low 1 × 10?9 m ssDNA-Cy5 fetal bovine serum (FBS). This work simple approach highly sensitive sources promising use POC diagnostics.